BIOCHEMISTRY

Biochemistry studies the structure and function of biological molecules including proteins, nucleic acids, carbohydrates, and lipids. In practice, biochemistry employs techniques to purify and decipher the expression levels, activity, interactions, and functions of biological molecules, including chromatography, immunoblotting/Western blotting, enzymatic assays, and co-immunoprecipitation. Impactys provides a wide range of biochemistry solutions, including:

  • Protein Production, Purification, and Formulation
    • Protein production
      • Secreted or intracellular
      • Recombinant protein production (Baculovirus, bacterial, yeast, animal)
    • Protein purification
      • Salt fractionation
      • Chromatography (HPLC, liquid, gel filtration, affinity, ion exchange, reverse phase, hydrophobic)
    • Formulation development
  • Protein Analysis
    • Concentration
    • Electrophoresis (SDS-PAGE, non-denaturing, isoelectric focusing)
    • Chromatography (RP-HPLC, size exclusion)
    • Binding/ligand-receptor assays
    • Kd, Scatchard analysis
    • Amino acid sequencing and analysis
    • Carbohydrate analysis
    • Equilibrium dialysis
    • Solubility
    • Bioassays
  • Enzymatic and Immunochemical Assays
    • Enzymatic assay development
      • Colorimetric, fluorescent, radiometric
      • Substrate specificity
      • Inhibitor kinetics
    • High-throughput screening (HTS) assays
    • Enzyme stability
    • Enzyme-linked immunosorbent assay (ELISA)
    • Radioimmunoassay (RIA)
    • Immunohistochemistry
    • Immunoblotting/Western blotting
    • Immunoprecipitation
  • Other Biochemical Methods
    • Database characterization
    • Endotoxin
    • Centrifugation (ultra, gradient, high-speed)
    • Detergent solubility for membrane extraction