MOLECULAR BIOLOGY & GENETIC ENGINEERING

Molecular biology studies the composition, structure, and interactions of cellular molecules that carry out biological processes, including the molecular structure and function of genes and gene behavior in the context of a cell or organism (e.g. epigenetics). Genetic engineering uses molecular biological tools to modify the genetic makeup of an organism by gene targeting, nuclear transplantation, transfection of synthetic chromosomes, or viral insertion. Impactys provides a wide range of molecular biological & genetic engineering solutions, including:

  • Nucleic Acid Isolation
    • Prokaryrotic and eukaryotic (bacteria, yeast, plant, animal cell lines, primary tissue samples)
    • Total RNA, mRNA
    • Genomic and plasmid DNA
    • cDNA purification
  • RNA Analysis
    • Conventional reverse-transcription PCR
    • Quantitative reverse-transcription PCR
    • Northern blotting
    • Dot/slot blotting
    • In situ hybridization
    • Rapid amplification of cDNA ends (RACE)
    • RNase protection assays
  • Gene Expression & Cloning
    • Restriction mapping
    • DNA subcloning
    • Genomic libraries
    • cDNA libraries
  • Gene Reporter Assays
    • CAT assays
    • Luciferase assays
    • Fluorescence assays
    • Chemiluminescence assays
    • Custom assays
  • Genetic Engineering
    • DNA subcloning
    • Site-directed mutagenesis (SDM)
    • Transcription activator-like effector nucleases (TALEN)
    • Clustered regularly interspaced short palindromic repeats (CRISPR)